Tuesday, October 17, 2017
Project co-funded by the European Commission in the framework of the 2nd Health Programme
Specific skin requirements Minimize

 

67. What is the maximum age limit for skin donors?

 

o

65 years

o

there is no maximum age limit

o

75 years

o

85 years for men, 95 years for women

 

 

68.  Would you recommend allograft skin of a parent for resurfacing large burn areas in children? Why (not)?

 

o

yes, it can overcome skin allograft shortage when the use of cadaver skin is avoided for cultural reasons

o

yes, it can overcome skin allograft shortage when the use of cadaver skin is avoided for alleged safety reasons (e.g. HIV transmission)

o

no, the skin of a parent is not necessarily compatible with his child

o

no, this practice is not recommended since it does not necessarily increase the biosecurity and results in an unnecessary medical risk with regard to the donor as well as an unnecessary cost to society

 

 

69. Tick the skin specific donor exclusion criteria

 

o

implantation of dura mater allograft

o

auto-immune dermatoses

o

malignant tumors of the anterior segment

o

dermal mucinosis

 

 

70.  Is human skin (or it’s derived products) used in vanity and cosmetic procedures?

 

o

no, it is illegal to use skin in vanity and cosmetic procedures

o

no, the use of human skin products in vanity or cosmetic procedures offers no advantages over the use of animal (e.g. porcine) skin products 

o

yes, some skin-based products are used, off-label and without explicit donor consent, in vanity procedures such as lip and –penis widening

o

yes, the use of human skin products in vanity and cosmetic procedures is common and widely accepted today

 

 

71.  What is the maximum post mortem time for skin procurement?

 

o

48 h, provided that the donor was refrigerated within 12 h after death and the blood samples for donor screening were collected and processed within 24 h after death

o

48 h, provided that the donor was refrigerated within 12 h after death and the blood samples for donor screening were collected and processed within 24 h after death

o

24 h, provided that the donor was refrigerated within 6 h after death and the blood samples for donor screening were collected and processed within 12 h after death

o

48 h, provided that the donor was refrigerated within 6 h after death and the blood samples for donor screening were collected and processed within 24 h after death

 

 

72. What is an appropriate thickness for partial thickness skin allografts?

 

o

0.5 – 1.0 nm

o

200 – 1000 µm

o

0.2 – 1.0 mm

o

0.5 – 1.0 cm

 

 

73. Why are skin donor sites treated with anti-bacterials prior to recovery of skin allografts and why are the recovered skin allografts often incubated in an antibiotic cocktail prior to processing?

 

o

the dermatome used for skin retrieval is not sterile

o

superficial decontamination of the skin donor sites is not indefectible

o

skin is often retrieved from non-heart-beating donors

 

o

in contrast to most harvested tissues (e.g. musculoskeletal tissue and heart valves), skin is inherently colonised by microorganisms and thus non-sterile at harvesting

 

 

74. Where should one look for when choosing a skin decontamination or preservation process?

 

o

the efficiency of the decontamination or preservation process

o

maintaining clinically relevant properties (e.g. viabilty) of the skin

o

maintaining the immunogenicity of the skin

o

all the above

 

 

 

75. From which donor sites is it inappropriate to procure skin?

 

o

the inner thighs

o

the neck, face and other places which could be visible when people pay their last respects to the donor

o

the lower back

o

all the above

 

 

76. Where should one pay specific attention to when ‘reconstructing’ a skin donor?

 

o

the donor sites should be decontaminated

o

measures should be taken to prevent fluid loss from the donor sites

o

the donor sites should be covered with artificial skin

o

all the above

 

      78. What are the objectives of the microbial evaluation of cryopreserved skin?

 

o

ensure a total absence of pathogens

o

provide proof of sterility

o

ensure the absence of substantial bioburdens of inherent skin commensals

o

prove the efficiency of the cryopreservation procedure

 

 

79. Which glycerol concentrations are normally used in the glycerolisation of skin?

 

o

5%

o

20-30%

o

45%

o

85-98%

 

 

80. Which statements regarding skin cryopreservation are true?

 

o

cryopreservation ensures a certain skin integrity and viability

o

cryopreservation significantly reduces immunogenicity

o

cryopreservation supports a certain viral, bacterial and fungal survival

o

cryopreservation eliminates skin viruses

 

 

81. Why would one remove the cells from donor skin?

 

o

it ensures a better preservation

o

it lowers the antigenicity of skin, which can be an advantage in certain clinical applications

o

it allows the skin to be processed in a grade C environment, instead of grade A

o

It allows the skin to be used in cosmetic procedures

 

 

.

81. The exclusion criteria for musculoskeletal tissue donors is not:

 

 

o

osteoporosis

o

osteopetrosis

o

diabetes

o

Paget’s disease

 

 

82. Musculoskeletal tissue retrieval from deceased donors shall occur no later than:

 

o

always 12 hours after death

o

24 hours after death if body was cooled 12 hours after death

o

48 hours after death if body was cooled 12 hours after death

o

always 48 hours after death

 

 

83. Musculoskeletal tissue retrieval from deceased donors shall occur no later than:

 

o

15-65 years for both sexes

o

15-45 years for women

o

25-45 years for both sexes

o

25- 45 years for women

 

 

     84.  For reconstruction of the donor’s body after procurement of musculoskeletal tissues could be used:

 

o

wooden stick

o

animal bone that match the size

o

cotton

o

paper towels

 

  

 

 

 

85. A storage time of cartilage for chondrocyte culture should not exceed:

 

o

1 day

o

2 days

o

3 days

o

4 days

 

86. Cancellous bone grafts cannot be prepared from:

 

o

epiphysis of distal femur

o

diaphysis of femur

o

vertebral body

o

iliac crest

 

87. The defeating procedure of bone tissue can be done by:

 

o

shaking machine with water

o

cHCl solution

o

lyophilisation

o

alcohol

 

88. Sterilisation of musculoskeletal grafts can be done by:

 

o

irradiation

o

autoclaving

o

demineralization

o

lyophilisation

 

89. Musculoskeletal grafts can be stored at room temperature after:

 

o

demineralization

o

DMSO conservation

o

lyophilisation

o

three layers vacuum packaging

 

90. Cultured chondrocytes should be transported at temperatures:

 

o

2-8°C

o

10-15°C

o

20-25°C

o

-20°C

 

Print  
A3f Engine ::Terms Of Use::Privacy Statement